Hoevenaars FP (1), van Schothorst EM (1), Horakova O (2), Voigt A (3), Rossmeisl M (2), Pico C (4),
Caimari A (5), Kopecky J (2), Klaus S (3), Keijer J (1)
Genes Nutr. 2012 Jan;7(3):399-404.

(1) Department of Human and Animal Physiology, Wageningen University, Wageningen, The Netherlands
(2) Department of Adipose Tissue Biology, Institute of Physiology Academy of Sciences of the Czech Republic v.v.i., Prague, Czech Republic
(3) Group of Energy Metabolism, German Institute of Human Nutrition in Potsdam, Nuthetal, Germany
(4) Molecular Biology, Nutrition and Biotechnology (Nutrigenomics), University of the Balearic Islands (UIB) and CIBER fisiopatología de la Obesidad y Nutrición (CIBEROBN), Palma de Mallorca, Spain
(5) Centre Tecnologic de Nutrició i Salut (CTNS), TECNIO, CEICS, Reus, Spain


Experimental replication is fundamental for practicing science. To reduce variability, it is essential to control sources of variation as much as possible. Diet is an important factor that can influence many processes and functional outcomes in studies performed with rodent models. This is especially true for, but not limited to, nutritional studies. To compare functional effects of different nutrients, it is important to use standardized, semi-purified diets. Here, we propose and describe a standard reference diet, the BIOCLAIMS standard diet. The diet is AIN-93 based, but further defined with dietary and experimental requirements taken into account that allow for experiments with bioactive food components and natural (non-expensive) labeling. This diet will be implemented by two European research consortia, Mitofood and BIOCLAIMS, to ensure inter-laboratory comparability.

DOI: 10.1007/s12263-011-0262-6

Event date: 07/01/2012

Publication date: 01/06/2012